Hotspot

ycf1

IRa · 137048-144028 · 6981 bp

Back to Codonopsis

Pi 0.0205

MarkerSeek score 60.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRa
length_bp: 6981
variable_sites: 324
pi: 0.0205
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATACTCCCCAGAAAGCCCTT	GCATAGGTTGGGGTTTGGAA	50.0	50.0	58.0	58.1	914-1046 bp	1.0	91.3
ycf1_p2	CCAAATCCTCAGACCACTCG	GCATAGGTTGGGGTTTGGAA	55.0	50.0	58.0	58.1	713-845 bp	1.0	91.3
ycf1_p3	ACCACTCGTTCTGTTGCAAT	GCATAGGTTGGGGTTTGGAA	45.0	50.0	58.0	58.1	701-833 bp	1.0	91.2
ycf1_p4	TCCCCAGAAAGCCCTTAGAA	GCATAGGTTGGGGTTTGGAA	50.0	50.0	57.9	58.1	910-1042 bp	1.0	91.0
ycf1_p5	ATACTCCCCAGAAAGCCCTT	TCAGCACGGGGTAATCAAAG	50.0	50.0	58.0	57.9	1133-1223 bp	0.444444	64.1