Hotspot

clpP

LSC · 75832-77841 · 2010 bp

Back to Codonopsis

Pi 0.0367

MarkerSeek score 73.90

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2010
variable_sites: 195
pi: 0.0367
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	ATCTCATACGGCTCCTCGAA	TCAGAATTACCACCCAGGGT	50.0	50.0	58.0	57.9	2557-2564 bp	0.333333	53.8
clpP_p2	AAATCTAAGGACCCGTTCGC	TCAGAATTACCACCCAGGGT	50.0	50.0	58.0	57.9	2527-2534 bp	0.333333	53.8
clpP_p3	ATCTCATACGGCTCCTCGAA	CTTCATGAAGCCACTCGGAA	50.0	50.0	58.0	57.9	2743-2750 bp	0.333333	53.4
clpP_p4	TCTAAGGACCCGTTCGCTAT	TCAGAATTACCACCCAGGGT	50.0	50.0	57.9	57.9	2524-2531 bp	0.333333	53.4
clpP_p5	CGCCACAAATTCCATCCCTA	TCAGAATTACCACCCAGGGT	50.0	50.0	57.9	57.9	2695-2702 bp	0.222222	48.4