Hotspot

trnI-CAU-rpl2

IRa · 167102-168191 · 1090 bp

Back to Codonopsis

Pi 0.0298

MarkerSeek score 76.00

Primer available yes

Label trnI-CAU-rpl2

Feature Metadata

label_name: trnI-CAU-rpl2
feature_type: igs
region: IRa
length_bp: 1090
variable_sites: 79
pi: 0.0298
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-rpl2_p1	CCCTAGTACGAGGATCCCTC	ACTGTCTACGGTTCCATTGC	60.0	50.0	57.8	57.9	742-1248 bp	1.0	87.6
trnI-CAU-rpl2_p2	CACCCTAGTACGAGGATCCC	ACTGTCTACGGTTCCATTGC	60.0	50.0	58.4	57.9	744-1250 bp	1.0	86.2
trnI-CAU-rpl2_p3	CCCTCTTAAGCATCCATGGC	ACTGTCTACGGTTCCATTGC	55.0	50.0	58.4	57.9	727-1233 bp	1.0	86.2
trnI-CAU-rpl2_p4	TGGCGAATTCGTAGGTTCAA	ACTGTCTACGGTTCCATTGC	45.0	50.0	57.5	57.9	679-1185 bp	1.0	85.9
trnI-CAU-rpl2_p5	TTGGCGAATTCGTAGGTTCA	ACTGTCTACGGTTCCATTGC	45.0	50.0	57.5	57.9	680-1186 bp	1.0	85.9