Hotspot

rpl32-trnL-UAG

SSC · 127045-127447 · 403 bp

Back to Codonopsis

Pi 0.0345
MarkerSeek score 71.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
403
variable_sites
37
pi
0.0345
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.96
markerseek_score
71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCTTTGGCATTGGGGAAATC AGCGTGTCTACCGATTTCAC 50.0 50.0 58.0 58.0 499-872 bp 0.777778 75.9
rpl32-trnL-UAG_p2 GCTTTGGCATTGGGGAAATC CAGCGTGTCTACCGATTTCA 50.0 50.0 58.0 58.0 500-873 bp 0.777778 75.9
rpl32-trnL-UAG_p3 GCTTTGGCATTGGGGAAATC AGAGGATGGTATATGCCGCT 50.0 50.0 58.0 58.1 563-936 bp 0.777778 75.1
rpl32-trnL-UAG_p4 GCTTTGGCATTGGGGAAATC AAAAGGCCCGAAGTAGCAAA 50.0 45.0 58.0 58.0 1026-1466 bp 0.777778 74.6
rpl32-trnL-UAG_p5 GCTTTGGCATTGGGGAAATC AATGACTAGAAGTGCGGCTG 50.0 50.0 58.0 58.0 1162-1602 bp 0.777778 74.5