Hotspot

ndhF-rpl32

SSC · 125913-126885 · 973 bp

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Pi 0.0313
MarkerSeek score 74.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
973
variable_sites
87
pi
0.0313
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.94
markerseek_score
74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GCATATTGATATGTCTGTTCCA AGCCCTTCCCTTTCCAAATC 36.4 50.0 54.1 57.8 1007-1122 bp 1.0 50.7
ndhF-rpl32_p2 GCATATTGATATGTCTGTTCCA GAGCCCTTCCCTTTCCAAAT 36.4 50.0 54.1 57.8 1008-1123 bp 1.0 50.7
ndhF-rpl32_p3 GCATATTGATATGTCTGTTCCA GCCCTTCCCTTTCCAAATCT 36.4 50.0 54.1 57.8 1006-1121 bp 1.0 50.7
ndhF-rpl32_p4 GCATATTGATATGTCTGTTCCAT AGCCCTTCCCTTTCCAAATC 34.8 50.0 54.6 57.8 1007-1122 bp 1.0 50.7
ndhF-rpl32_p5 GCATATTGATATGTCTGTTCCAT GCCCTTCCCTTTCCAAATCT 34.8 50.0 54.6 57.8 1006-1121 bp 1.0 50.7