Hotspot

rpl2-trnI-CAU

IRb · 86299-87388 · 1090 bp

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Pi 0.03

MarkerSeek score 76.10

Primer available yes

Label rpl2-trnI-CAU

Feature Metadata

label_name: rpl2-trnI-CAU
feature_type: igs
region: IRb
length_bp: 1090
variable_sites: 80
pi: 0.03
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-trnI-CAU_p1	ACTGTCTACGGTTCCATTGC	CCCTAGTACGAGGATCCCTC	50.0	60.0	57.9	57.8	742-1248 bp	1.0	87.7
rpl2-trnI-CAU_p2	ACTGTCTACGGTTCCATTGC	CCCTCTTAAGCATCCATGGC	50.0	55.0	57.9	58.4	727-1233 bp	1.0	86.3
rpl2-trnI-CAU_p3	ACTGTCTACGGTTCCATTGC	CACCCTAGTACGAGGATCCC	50.0	60.0	57.9	58.4	744-1250 bp	1.0	86.2
rpl2-trnI-CAU_p4	ACTGTCTACGGTTCCATTGC	TGGCGAATTCGTAGGTTCAA	50.0	45.0	57.9	57.5	679-1185 bp	1.0	86.0
rpl2-trnI-CAU_p5	ACTGTCTACGGTTCCATTGC	TTGGCGAATTCGTAGGTTCA	50.0	45.0	57.9	57.5	680-1186 bp	1.0	86.0