Hotspot

rpl36-rps8

LSC · 79779-80555 · 777 bp

Back to Codonopsis

Pi 0.0703

MarkerSeek score 85.30

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 777
variable_sites: 135
pi: 0.0703
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 85.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	TTTATCCGGACTTGTGCTCG	AGTCGACCTGGTCTACGAAT	50.0	50.0	58.0	57.9	1410-2223 bp	1.0	90.1
rpl36-rps8_p2	TTTATCCGGACTTGTGCTCG	GAAGTCGAGTTTTTGGTGCG	50.0	50.0	58.0	58.0	1889-2714 bp	1.0	89.8
rpl36-rps8_p3	GGCGATCCTTTTCGGTTACT	GAAGTCGAGTTTTTGGTGCG	50.0	50.0	58.0	58.0	1817-2642 bp	1.0	89.8
rpl36-rps8_p4	GGCGATCCTTTTCGGTTACT	AAAACGGATCAGTCGACCTG	50.0	50.0	58.0	57.9	1360-2111 bp	0.666667	76.1
rpl36-rps8_p5	TTTATCCGGACTTGTGCTCG	AAAACGGATCAGTCGACCTG	50.0	50.0	58.0	57.9	1432-2183 bp	0.666667	75.8