Hotspot

rpoC2-atpH

LSC · 36869-38003 · 1135 bp

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Pi 0.222
MarkerSeek score 83.60
Primer available yes
Label rpoC2-atpH

Feature Metadata

label_name
rpoC2-atpH
feature_type
igs
region
LSC
length_bp
1135
variable_sites
537
pi
0.222
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC2-atpH_p1 TTTTTCACCCGGAGAACTGG CTCGCGATACCTTCTACAGC 50.0 55.0 58.0 58.0 1678-2852 bp 1.0 91.5
rpoC2-atpH_p2 GAACTGGTTGGATTGTTGCG CTCGCGATACCTTCTACAGC 50.0 55.0 57.9 58.0 1665-2839 bp 1.0 91.3
rpoC2-atpH_p3 TTTTTCACCCGGAGAACTGG GATTACGAAGGGGAAGGCTG 50.0 55.0 58.0 58.0 1983-2833 bp 0.888889 87.0
rpoC2-atpH_p4 ACGCAAGGTCTTCCAAAAGT CTCGCGATACCTTCTACAGC 45.0 55.0 57.9 58.0 1977-2827 bp 0.888889 86.8
rpoC2-atpH_p5 GAACTGGTTGGATTGTTGCG GATTACGAAGGGGAAGGCTG 50.0 55.0 57.9 58.0 1970-2820 bp 0.888889 86.8