Hotspot

trnT-GGU-trnM-CAU

LSC · 21354-21782 · 429 bp

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Pi 0.0355
MarkerSeek score 74.40
Primer available yes
Label trnT-GGU-trnM-CAU

Feature Metadata

label_name
trnT-GGU-trnM-CAU
feature_type
igs
region
LSC
length_bp
429
variable_sites
40
pi
0.0355
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-trnM-CAU_p1 ACGCCTTACCATGGTGTTAC TCGGTCAACAAACTGAGCAA 50.0 45.0 57.9 58.0 1143-1180 bp 1.0 88.0
trnT-GGU-trnM-CAU_p2 AACAGAAGGGAGGAGGTCAA TCGGTCAACAAACTGAGCAA 50.0 45.0 57.9 58.0 1455-1492 bp 1.0 86.5
trnT-GGU-trnM-CAU_p3 CTTGCGGAGTCCAAAGAACT TCGGTCAACAAACTGAGCAA 50.0 45.0 58.1 58.0 1490-1512 bp 0.444444 58.9
trnT-GGU-trnM-CAU_p4 GCGGAGTCCAAAGAACTGAA TCGGTCAACAAACTGAGCAA 50.0 45.0 58.1 58.0 1487-1509 bp 0.444444 58.9
trnT-GGU-trnM-CAU_p5 TCTTGCGGAGTCCAAAGAAC TCGGTCAACAAACTGAGCAA 50.0 45.0 58.1 58.0 1491-1513 bp 0.444444 58.8