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rps16-trnT-UGU

LSC · 5054-6307 · 1254 bp

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Pi 0.028

MarkerSeek score 73.10

Primer available yes

Label rps16-trnT-UGU

Feature Metadata

label_name: rps16-trnT-UGU
feature_type: igs
region: LSC
length_bp: 1254
variable_sites: 95
pi: 0.028
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnT-UGU_p1	CGGTGCTTTTTACCACATCG	CTAACCACGGAGCTAAGCAG	50.0	55.0	57.8	58.1	1275-1446 bp	1.0	87.1
rps16-trnT-UGU_p2	CGGTGCTTTTTACCACATCG	CTCTAACCACGGAGCTAAGC	50.0	55.0	57.8	57.8	1277-1448 bp	1.0	86.0
rps16-trnT-UGU_p3	CGGGATCGAACATCAATTGC	CTAACCACGGAGCTAAGCAG	50.0	55.0	57.6	58.1	1314-1485 bp	1.0	85.7
rps16-trnT-UGU_p4	ATAAGTCGCACGGTGCTTTT	CTAACCACGGAGCTAAGCAG	45.0	55.0	58.5	58.1	1285-1456 bp	1.0	85.1
rps16-trnT-UGU_p5	CGGGATCGAACATCAATTGC	CTCTAACCACGGAGCTAAGC	50.0	55.0	57.6	57.8	1316-1487 bp	1.0	84.6