Hotspot

ycf3

Genome · 11012-11859 · 848 bp

Pi 0.188

MarkerSeek score 83.60

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: Genome
length_bp: 848
variable_sites: 260
pi: 0.188
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	GGGAAAATTTTACCACGCCG	AAAACAATCCAGTCGGCCTT	50.0	45.0	58.0	58.0	1145 bp	0.25	49.8
ycf3_p2	GGGAAAATTTTACCACGCCG	AAACAATCCAGTCGGCCTTT	50.0	45.0	58.0	58.0	1144 bp	0.25	49.8
ycf3_p3	GGGAAAATTTTACCACGCCG	TCCAGTCGGCCTTTCTTCTA	50.0	50.0	58.0	58.1	1138 bp	0.25	49.3
ycf3_p4	GGGAAAATTTTACCACGCCG	CCCTTCATCGTCTTTGAGCA	50.0	50.0	58.0	57.9	1254 bp	0.25	49.1
ycf3_p5	GGGAAAATTTTACCACGCCG	TTTTCGCAGTTTAACACGCC	50.0	45.0	58.0	57.9	1272 bp	0.25	49.0