Hotspot

rps19

Genome · 70634-71446 · 813 bp

Back to Codium

Pi 0.123
MarkerSeek score 83.70
Primer available yes
Label rps19

Feature Metadata

label_name
rps19
feature_type
gene
region
Genome
length_bp
813
variable_sites
165
pi
0.123
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19_p1 CGGCTCCATTTAAACGACCT ATAAAAGACCCTCGGTTCGC 50.0 50.0 58.3 58.0 1589 bp 0.25 47.7
rps19_p2 TCGGCTCCATTTAAACGACC ATAAAAGACCCTCGGTTCGC 50.0 50.0 58.3 58.0 1590 bp 0.25 47.7
rps19_p3 CGGCTCCATTTAAACGACCT CGCGGTTCAGTTATGAATGC 50.0 50.0 58.3 57.9 1572 bp 0.25 47.1
rps19_p4 TCGGCTCCATTTAAACGACC CGCGGTTCAGTTATGAATGC 50.0 50.0 58.3 57.9 1573 bp 0.25 47.1
rps19_p5 CGGCTCCATTTAAACGACCT ATCGAGGAGGAGGGCATAAA 50.0 50.0 58.3 57.9 2075 bp 0.25 47.0