Hotspot

rpoC2

Genome · 15385-19089 · 3705 bp

Back to Codium

Pi 0.22
MarkerSeek score 77.30
Primer available yes
Label rpoC2

Feature Metadata

label_name
rpoC2
feature_type
gene
region
Genome
length_bp
3705
variable_sites
1324
pi
0.22
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC2_p1 TGCCTTCCATACTAAGCAAGG TGGAACAATTTGGATCGCCA 47.6 45.0 57.7 58.1 1291 bp 0.25 39.1
rpoC2_p2 TGCCTTCCATACTAAGCAAGG ATCGCCAAAGTGTTTGCATC 47.6 45.0 57.7 57.6 1278 bp 0.25 36.9
rpoC2_p3 TGCCTTCCATACTAAGCAAGG GATCGCCAAAGTGTTTGCAT 47.6 45.0 57.7 57.6 1279 bp 0.25 36.9
rpoC2_p4 GCCTTCCATACTAAGCAAGGA TGGAACAATTTGGATCGCCA 47.6 45.0 57.4 58.1 1290 bp 0.25 36.8
rpoC2_p5 TGGAGTTGTGGAGTCAGAAA TGGAACAATTTGGATCGCCA 45.0 45.0 56.3 58.1 867 bp 0.25 35.8