Hotspot

ccs1

Genome · 28282-29423 · 1142 bp

Pi 0.199

MarkerSeek score 82.30

Primer available yes

Label ccs1

Feature Metadata

label_name: ccs1
feature_type: gene
region: Genome
length_bp: 1142
variable_sites: 371
pi: 0.199
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccs1_p1	GCTTTTTCACGAACAGAGGC	TGGATCTAATGCCCCAAACG	50.0	50.0	58.0	57.9	2250 bp	0.25	49.3
ccs1_p2	GCTTTTTCACGAACAGAGGC	GATCTAATGCCCCAAACGGT	50.0	50.0	58.0	57.9	2248 bp	0.25	49.3
ccs1_p3	GCTTTTTCACGAACAGAGGC	CGGCTTTTTCAAAACCAGCA	50.0	45.0	58.0	58.1	1918 bp	0.25	49.2
ccs1_p4	GCTTTTTCACGAACAGAGGC	TCAATCGCTAATGCTCGGAC	50.0	50.0	58.0	58.1	2204 bp	0.25	48.5
ccs1_p5	GCTTTTTCACGAACAGAGGC	GCCCCAAACGGTTCATCTAA	50.0	50.0	58.0	58.2	2240 bp	0.25	48.3