Hotspot

rns-trnN

Genome · 1546-2086 · 541 bp

Back to Codium

Pi 0.392
MarkerSeek score 57.40
Primer available yes
Label rns-trnN

Feature Metadata

label_name
rns-trnN
feature_type
igs
region
Genome
length_bp
541
variable_sites
167
pi
0.392
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.00
markerseek_score
57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rns-trnN_p1 AGAGCTAGTGACTCAAGCGA CTCTACCATTGAGCTACTAAGGA 50.0 43.5 58.2 56.6 517-743 bp 1.0 55.6
rns-trnN_p2 GTAACCGTACTGGAAGGTGC CTCTACCATTGAGCTACTAAGGA 55.0 43.5 58.3 56.6 485-711 bp 1.0 54.8
rns-trnN_p3 AGGTAACCGTACTGGAAGGT CTCTACCATTGAGCTACTAAGGA 50.0 43.5 57.7 56.6 487-713 bp 1.0 54.5
rns-trnN_p4 AGAGCTAGTGACTCAAGCGA TCTACCATTGAGCTACTAAGGA 50.0 40.9 58.2 55.5 516-742 bp 1.0 54.3
rns-trnN_p5 GCTAGTGACTCAAGCGAAGT CTCTACCATTGAGCTACTAAGGA 50.0 43.5 57.6 56.6 514-740 bp 1.0 54.2