primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rns-trnN_p1	rns-trnN	1	AGAGCTAGTGACTCAAGCGA	CTCTACCATTGAGCTACTAAGGA	20	23	50.0	43.5	58.2	56.6	2.0	0.0	4.545057	1546	2086	517-743	1.000000	55.6
rns-trnN_p2	rns-trnN	2	GTAACCGTACTGGAAGGTGC	CTCTACCATTGAGCTACTAAGGA	20	23	55.0	43.5	58.3	56.6	0.0	0.0	4.654342	1546	2086	485-711	1.000000	54.8
rns-trnN_p3	rns-trnN	3	AGGTAACCGTACTGGAAGGT	CTCTACCATTGAGCTACTAAGGA	20	23	50.0	43.5	57.7	56.6	10.2	0.0	4.688458	1546	2086	487-713	1.000000	54.5
rns-trnN_p4	rns-trnN	4	AGAGCTAGTGACTCAAGCGA	TCTACCATTGAGCTACTAAGGA	20	22	50.0	40.9	58.2	55.5	2.0	0.0	4.712298	1546	2086	516-742	1.000000	54.3
rns-trnN_p5	rns-trnN	5	GCTAGTGACTCAAGCGAAGT	CTCTACCATTGAGCTACTAAGGA	20	23	50.0	43.5	57.6	56.6	0.0	0.0	4.728455	1546	2086	514-740	1.000000	54.2
ftsH_p1	ftsH	1	AGTATTTACATAGGCTTCTTCG	ACACAAAATGTTGGCCTGAC	22	20	36.4	45.0	53.5	57.1	0.0	3.3	7.400008	6085	6204	703-703	0.250000	10.0
ftsH_p2	ftsH	2	AGTATTTACATAGGCTTCTTCG	GACACAAAATGTTGGCCTGA	22	20	36.4	45.0	53.5	56.8	0.0	3.3	7.686830	6085	6204	704-704	0.250000	10.0
ftsH_p3	ftsH	3	AGTATTTACATAGGCTTCTTCG	AGACACAAAATGTTGGCCTG	22	20	36.4	45.0	53.5	56.8	0.0	3.3	7.695483	6085	6204	705-705	0.250000	10.0
ftsH_p4	ftsH	4	AGTATTTACATAGGCTTCTTCG	CACAAAATGTTGGCCTGACT	22	20	36.4	45.0	53.5	56.8	0.0	3.3	7.695483	6085	6204	702-702	0.250000	10.0
ftsH_p5	ftsH	5	AGTATTTACATAGGCTTCTTCG	GACACAAAATGTTGGCCTGAC	22	21	36.4	47.6	53.5	58.3	0.0	3.3	7.768400	6085	6204	704-704	0.250000	10.0
ycf3_p1	ycf3	1	GGGAAAATTTTACCACGCCG	AAAACAATCCAGTCGGCCTT	20	20	50.0	45.0	58.0	58.0	24.9	0.0	0.019289	11012	11859	1145-1145	0.250000	49.8
ycf3_p2	ycf3	2	GGGAAAATTTTACCACGCCG	AAACAATCCAGTCGGCCTTT	20	20	50.0	45.0	58.0	58.0	24.9	0.0	0.019289	11012	11859	1144-1144	0.250000	49.8
ycf3_p3	ycf3	3	GGGAAAATTTTACCACGCCG	TCCAGTCGGCCTTTCTTCTA	20	20	50.0	50.0	58.0	58.1	24.9	0.0	0.081644	11012	11859	1138-1138	0.250000	49.3
ycf3_p4	ycf3	4	GGGAAAATTTTACCACGCCG	CCCTTCATCGTCTTTGAGCA	20	20	50.0	50.0	58.0	57.9	24.9	0.0	0.113807	11012	11859	1254-1254	0.250000	49.1
ycf3_p5	ycf3	5	GGGAAAATTTTACCACGCCG	TTTTCGCAGTTTAACACGCC	20	20	50.0	45.0	58.0	57.9	24.9	0.0	0.122844	11012	11859	1272-1272	0.250000	49.0
rpoC2_p1	rpoC2	1	TGCCTTCCATACTAAGCAAGG	TGGAACAATTTGGATCGCCA	21	20	47.6	45.0	57.7	58.1	0.0	0.8	1.365221	17039	17158	1291-1291	0.250000	39.1
rpoC2_p2	rpoC2	2	TGCCTTCCATACTAAGCAAGG	ATCGCCAAAGTGTTTGCATC	21	20	47.6	45.0	57.7	57.6	0.0	0.0	1.634230	17039	17158	1278-1278	0.250000	36.9
rpoC2_p3	rpoC2	3	TGCCTTCCATACTAAGCAAGG	GATCGCCAAAGTGTTTGCAT	21	20	47.6	45.0	57.7	57.6	0.0	0.0	1.634230	17039	17158	1279-1279	0.250000	36.9
rpoC2_p4	rpoC2	4	GCCTTCCATACTAAGCAAGGA	TGGAACAATTTGGATCGCCA	21	20	47.6	45.0	57.4	58.1	0.0	0.8	1.644131	17039	17158	1290-1290	0.250000	36.8
rpoC2_p5	rpoC2	5	TGGAGTTGTGGAGTCAGAAA	TGGAACAATTTGGATCGCCA	20	20	45.0	45.0	56.3	58.1	0.0	0.8	1.771976	17039	17158	867-867	0.250000	35.8
ccs1_p1	ccs1	1	GCTTTTTCACGAACAGAGGC	TGGATCTAATGCCCCAAACG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.089275	28282	29423	2250-2250	0.250000	49.3
ccs1_p2	ccs1	2	GCTTTTTCACGAACAGAGGC	GATCTAATGCCCCAAACGGT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.090723	28282	29423	2248-2248	0.250000	49.3
ccs1_p3	ccs1	3	GCTTTTTCACGAACAGAGGC	CGGCTTTTTCAAAACCAGCA	20	20	50.0	45.0	58.0	58.1	0.0	8.5	0.104694	28282	29423	1918-1918	0.250000	49.2
ccs1_p4	ccs1	4	GCTTTTTCACGAACAGAGGC	TCAATCGCTAATGCTCGGAC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.184999	28282	29423	2204-2204	0.250000	48.5
ccs1_p5	ccs1	5	GCTTTTTCACGAACAGAGGC	GCCCCAAACGGTTCATCTAA	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.208962	28282	29423	2240-2240	0.250000	48.3
psbB_p1	psbB	1	TGTCCATAGAGTGCTTGTGT	GCCTGGAGTGACTTTTCCAT	20	20	45.0	50.0	56.5	57.8	0.0	0.0	1.728837	30208	32538	2940-2940	0.250000	36.2
psbB_p2	psbB	2	TGTCCATAGAGTGCTTGTGT	AAAAGTGTAGTTCCCCAGCC	20	20	45.0	50.0	56.5	57.7	0.0	0.0	1.810238	30208	32538	2957-2957	0.250000	35.5
psbB_p3	psbB	3	TGTCCATAGAGTGCTTGTGTT	GCCTGGAGTGACTTTTCCAT	21	20	42.9	50.0	57.2	57.8	0.0	0.0	1.976315	30208	32538	2940-2940	0.250000	34.2
psbB_p4	psbB	4	TTGTCCATAGAGTGCTTGTGT	GCCTGGAGTGACTTTTCCAT	21	20	42.9	50.0	57.2	57.8	0.0	0.0	1.976315	30208	32538	2941-2941	0.250000	34.2
psbB_p5	psbB	5	TGTCCATAGAGTGCTTGTGT	CAGCCTGGAGTGACTTTTCC	20	20	45.0	55.0	56.5	58.5	0.0	0.0	1.994132	30208	32538	2942-2942	0.250000	34.0
rbcL_p1	rbcL	1	CTCCGGTAAGATAGTCGGGT	CGTATGACTCCACAACCTGG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.031691	54036	54155	2418-2464	0.500000	70.2
rbcL_p2	rbcL	2	CTAGTGTTAGCGGTCCAACC	AACATTTCAAGGACCACCCC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.015652	54036	54155	2120-2120	0.250000	49.9
rbcL_p3	rbcL	3	CTAGTGTTAGCGGTCCAACC	CGTATGACTCCACAACCTGG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.016116	54036	54155	2437-2437	0.250000	49.9
rbcL_p4	rbcL	4	ACCTCCGGTAAGATAGTCGG	AACATTTCAAGGACCACCCC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.031227	54036	54155	2103-2103	0.250000	49.8
rbcL_p5	rbcL	5	CTCCGGTAAGATAGTCGGGT	AACATTTCAAGGACCACCCC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.031227	54036	54155	2101-2101	0.250000	49.8
orf276_p1	orf276	1	CTCCGGTAAGATAGTCGGGT	CGTATGACTCCACAACCTGG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.031691	53178	54008	2418-2464	0.500000	70.2
orf276_p2	orf276	2	CTAGTGTTAGCGGTCCAACC	AACATTTCAAGGACCACCCC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.015652	53178	54008	2120-2120	0.250000	49.9
orf276_p3	orf276	3	CTAGTGTTAGCGGTCCAACC	CGTATGACTCCACAACCTGG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.016116	53178	54008	2437-2437	0.250000	49.9
orf276_p4	orf276	4	ACCTCCGGTAAGATAGTCGG	AACATTTCAAGGACCACCCC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.031227	53178	54008	2103-2103	0.250000	49.8
orf276_p5	orf276	5	CTCCGGTAAGATAGTCGGGT	AACATTTCAAGGACCACCCC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.031227	53178	54008	2101-2101	0.250000	49.8
orf107-petG_p1	orf107-petG	1	AAAACGTCGCACTCGTGTAT	CAGGAAGGAGATGTGTCTGC	20	20	45.0	55.0	58.2	58.0	0.0	0.0	0.246717	54350	55709	2149-2149	0.250000	48.0
orf107-petG_p2	orf107-petG	2	AAAACGTCGCACTCGTGTAT	CCGATCTTGATCAGGCAACA	20	20	45.0	50.0	58.2	58.0	0.0	10.7	0.253781	54350	55709	1914-1914	0.250000	48.0
orf107-petG_p3	orf107-petG	3	AAAACGTCGCACTCGTGTAT	CTTTCCGATCAGGTGGCTAC	20	20	45.0	55.0	58.2	58.1	0.0	0.0	0.284036	54350	55709	1825-1825	0.250000	47.7
orf107-petG_p4	orf107-petG	4	AAAACGTCGCACTCGTGTAT	AATCTCCGTTTCACGAGTGG	20	20	45.0	50.0	58.2	57.9	0.0	3.2	0.305647	54350	55709	2033-2033	0.250000	47.6
orf107-petG_p5	orf107-petG	5	AAAACGTCGCACTCGTGTAT	GGTCTTCCGCACAACCTAAA	20	20	45.0	50.0	58.2	58.1	0.0	0.0	0.338475	54350	55709	2013-2013	0.250000	47.3
atpF_p1	atpF	1	CGCAATACCATCCCCAACTT	GTATTGCTCGTCAGCCTGAA	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.247438	57784	58658	1425-1425	0.250000	48.0
atpF_p2	atpF	2	CGCAATACCATCCCCAACTT	TTGGAATCGGTGCAAGAACA	20	20	50.0	45.0	58.2	58.0	0.0	0.0	0.263027	57784	58658	1595-1595	0.250000	47.9
atpF_p3	atpF	3	CGCAATACCATCCCCAACTT	ACTTGTTCCCGTACCCCTAA	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.265854	57784	58658	1760-1760	0.250000	47.9
atpF_p4	atpF	4	CGCAATACCATCCCCAACTT	TTACTTGTTCCCGTACCCCT	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.265854	57784	58658	1762-1762	0.250000	47.9
atpF_p5	atpF	5	CGCAATACCATCCCCAACTT	TCCCGTACCCCTAATGTTGT	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.281841	57784	58658	1754-1754	0.250000	47.7
rps2_p1	rps2	1	AGGGGTACGGGAACAAGTAA	GAACTAGTGACATTCCGCGT	20	20	50.0	50.0	58.0	58.0	0.0	4.9	0.036392	59928	60617	1939-1939	0.250000	49.7
rps2_p2	rps2	2	TTAGGGGTACGGGAACAAGT	GAACTAGTGACATTCCGCGT	20	20	50.0	50.0	58.0	58.0	0.0	4.9	0.036392	59928	60617	1941-1941	0.250000	49.7
rps2_p3	rps2	3	AGGGGTACGGGAACAAGTAA	TGAACTAGTGACATTCCGCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.037762	59928	60617	1940-1940	0.250000	49.7
rps2_p4	rps2	4	TTAGGGGTACGGGAACAAGT	TGAACTAGTGACATTCCGCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.037762	59928	60617	1942-1942	0.250000	49.7
rps2_p5	rps2	5	AGGGGTACGGGAACAAGTAA	CCCGGGGGTATTTTTAGAGC	20	20	50.0	55.0	58.0	58.0	0.0	14.7	0.051505	59928	60617	1497-1497	0.250000	49.6
rpl5_p1	rpl5	1	ATAAATGCGTCGTCCTGGTG	TTTCGGACCTATTGCTCGTG	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.352595	68243	69069	1200-1200	0.250000	47.2
rpl5_p2	rpl5	2	ATAAATGCGTCGTCCTGGTG	AATTACAAAACGTGCCGCTG	20	20	50.0	45.0	58.3	58.0	0.0	0.0	0.392901	68243	69069	1712-1712	0.250000	46.9
rpl5_p3	rpl5	3	ATAAATGCGTCGTCCTGGTG	TTGGTTAACCGCTCGACAAA	20	20	50.0	45.0	58.3	58.0	0.0	19.6	0.397185	68243	69069	1811-1811	0.250000	46.8
rpl5_p4	rpl5	4	CGTCCTGGTGTACTGATACG	TTTCGGACCTATTGCTCGTG	20	20	55.0	50.0	57.5	58.0	0.0	0.0	0.474779	68243	69069	1190-1190	0.250000	46.2
rpl5_p5	rpl5	5	ATAAATGCGTCGTCCTGGTG	TTGCAGTTGTCAAACCAGGT	20	20	50.0	45.0	58.3	58.2	0.0	0.0	0.502006	68243	69069	1650-1650	0.250000	46.0
rps19_p1	rps19	1	CGGCTCCATTTAAACGACCT	ATAAAAGACCCTCGGTTCGC	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.285785	70634	71446	1589-1589	0.250000	47.7
rps19_p2	rps19	2	TCGGCTCCATTTAAACGACC	ATAAAAGACCCTCGGTTCGC	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.290664	70634	71446	1590-1590	0.250000	47.7
rps19_p3	rps19	3	CGGCTCCATTTAAACGACCT	CGCGGTTCAGTTATGAATGC	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.356989	70634	71446	1572-1572	0.250000	47.1
rps19_p4	rps19	4	TCGGCTCCATTTAAACGACC	CGCGGTTCAGTTATGAATGC	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.361868	70634	71446	1573-1573	0.250000	47.1
rps19_p5	rps19	5	CGGCTCCATTTAAACGACCT	ATCGAGGAGGAGGGCATAAA	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.368991	70634	71446	2075-2075	0.250000	47.0
accD_p1	accD	1	AATTGCTGGAACACCGACTT	AATTCGACCGGTTGTTCCAA	20	20	45.0	45.0	58.0	58.0	0.0	21.5	0.048652	77135	78160	2297-2297	0.250000	49.6
accD_p2	accD	2	ACCAACAACCCTCGGTTTAC	AATTCGACCGGTTGTTCCAA	20	20	50.0	45.0	58.0	58.0	0.0	21.5	0.058427	77135	78160	1845-1845	0.250000	49.5
accD_p3	accD	3	CCAACAACCCTCGGTTTACA	AATTCGACCGGTTGTTCCAA	20	20	50.0	45.0	58.0	58.0	0.0	21.5	0.059816	77135	78160	1844-1844	0.250000	49.5
accD_p4	accD	4	TCCATCCATTTGAGCTGCTC	AATTCGACCGGTTGTTCCAA	20	20	50.0	45.0	58.0	58.0	22.4	21.5	0.078352	77135	78160	2207-2207	0.250000	49.4
accD_p5	accD	5	ATCCATTTGAGCTGCTCCAG	AATTCGACCGGTTGTTCCAA	20	20	50.0	45.0	57.9	58.0	25.6	21.5	0.084140	77135	78160	2204-2204	0.250000	49.3