Hotspot

ycf1

SSC · 123919-129240 · 5322 bp

Back to Cochlearia

Pi 0.201

MarkerSeek score 75.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5322
variable_sites: 1607
pi: 0.201
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TAACGAGTCGGCCTCAAAAA	TCATCTCAGAAGGCTCGACT	45.0	50.0	57.5	57.9	80-1269 bp	0.75	76.7
ycf1_p2	AATAACGAGTCGGCCTCAAA	TCATCTCAGAAGGCTCGACT	45.0	50.0	57.2	57.9	80-1271 bp	0.75	74.9
ycf1_p3	ATAACGAGTCGGCCTCAAAA	TCATCTCAGAAGGCTCGACT	45.0	50.0	57.2	57.9	80-1270 bp	0.75	74.9
ycf1_p4	TAACGAGTCGGCCTCAAAAA	CAATTTCCCGAGTGGTCTGA	45.0	50.0	57.5	57.5	80-1449 bp	0.75	74.0
ycf1_p5	TAACGAGTCGGCCTCAAAAA	TCAATTTCCCGAGTGGTCTG	45.0	50.0	57.5	57.5	80-1450 bp	0.75	74.0