Hotspot

ndhI

SSC · 119273-119755 · 483 bp

Back to Cochlearia

Pi 0.266

MarkerSeek score 68.40

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 483
variable_sites: 193
pi: 0.266
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	TTGTAGTCCAAACAACCCCG	GAATGCGAGAGGTTACCGTT	50.0	50.0	58.0	58.0	80-1724 bp	0.75	81.7
ndhI_p2	TTGTAGTCCAAACAACCCCG	GCGAGAGGTTACCGTTTGAT	50.0	50.0	58.0	58.0	80-1720 bp	0.75	81.7
ndhI_p3	AGAAGACCGGATCCCAGAAA	GAATGCGAGAGGTTACCGTT	50.0	50.0	58.0	58.0	80-1401 bp	0.75	81.6
ndhI_p4	AGAAGACCGGATCCCAGAAA	GCGAGAGGTTACCGTTTGAT	50.0	50.0	58.0	58.0	80-1397 bp	0.75	81.6
ndhI_p5	ACTAGAAGACCGGATCCCAG	GCGAGAGGTTACCGTTTGAT	55.0	50.0	57.9	58.0	80-1400 bp	0.75	81.4