Hotspot

ndhG

SSC · 118381-118911 · 531 bp

Back to Cochlearia

Pi 0.264

MarkerSeek score 80.70

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 211
pi: 0.264
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	GCCGCTGCAATTGCTATAAC	TCTGCCTGTTGTTGATTGGA	50.0	45.0	58.2	57.3	80-1629 bp	0.75	74.8
ndhG_p2	TGATGCGCGTTGATTTTCTG	TCTGCCTGTTGTTGATTGGA	45.0	45.0	57.8	57.3	80-1694 bp	0.75	74.8
ndhG_p3	GATGCGCGTTGATTTTCTGT	TCTGCCTGTTGTTGATTGGA	45.0	45.0	57.8	57.3	80-1693 bp	0.75	74.8
ndhG_p4	AGCCGCTGCAATTGCTATAA	TCTGCCTGTTGTTGATTGGA	45.0	45.0	58.3	57.3	80-1630 bp	0.75	74.2
ndhG_p5	GATTGATTGATGCGCGTTGA	TCTGCCTGTTGTTGATTGGA	45.0	45.0	57.5	57.3	80-1701 bp	0.75	73.1