Hotspot

ndhD

SSC · 115647-117170 · 1524 bp

Back to Cochlearia

Pi 0.282

MarkerSeek score 71.40

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1524
variable_sites: 644
pi: 0.282
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CCATCGGCATTCTTTCAGGA	CTCAATGTGTTCGAGCATGC	50.0	50.0	58.0	57.8	80-2350 bp	0.75	80.3
ndhD_p2	CCATCGGCATTCTTTCAGGA	AAGAACCGAGGACTGTGTTG	50.0	50.0	58.0	57.8	80-2265 bp	0.75	79.7
ndhD_p3	CCATCGGCATTCTTTCAGGA	CAAGAACCGAGGACTGTGTT	50.0	50.0	58.0	57.8	80-2266 bp	0.75	79.7
ndhD_p4	CCATCGGCATTCTTTCAGGA	GCATGCCCTACAGACGTATT	50.0	50.0	58.0	57.8	80-2336 bp	0.75	79.7
ndhD_p5	CCATCGGCATTCTTTCAGGA	GTTATAGCAATTGCAGCGGC	50.0	50.0	58.0	58.2	80-2752 bp	0.75	79.7