Hotspot

ndhA

SSC · 119842-122032 · 2191 bp

Back to Cochlearia

Pi 0.273

MarkerSeek score 79.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2191
variable_sites: 898
pi: 0.273
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CAGCTCGTAGGGTTTGTTGA	AGTTGAAGCTCCAAAAGGGG	50.0	50.0	57.8	58.0	80-2532 bp	0.75	80.7
ndhA_p2	AGCTCGTAGGGTTTGTTGAC	AGTTGAAGCTCCAAAAGGGG	50.0	50.0	57.8	58.0	80-2531 bp	0.75	80.7
ndhA_p3	CAGCTCGTAGGGTTTGTTGA	GTTGAAGCTCCAAAAGGGGA	50.0	50.0	57.8	58.0	80-2531 bp	0.75	80.7
ndhA_p4	AGCTCGTAGGGTTTGTTGAC	GTTGAAGCTCCAAAAGGGGA	50.0	50.0	57.8	58.0	80-2530 bp	0.75	80.6
ndhA_p5	CAGCTCGTAGGGTTTGTTGA	ATGGAAAATACGACCACCGG	50.0	50.0	57.8	58.0	80-2466 bp	0.75	80.4