Hotspot

ndhE-ndhG

SSC · 118113-118380 · 268 bp

Back to Cochlearia

Pi 0.302

MarkerSeek score 68.00

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 268
variable_sites: 121
pi: 0.302
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	GCATGCTCGAACACATTGAG	CGGGGTTGTTTGGACTACAA	50.0	50.0	57.8	58.0	80-1054 bp	0.75	80.4
ndhE-ndhG_p2	GCATGCTCGAACACATTGAG	TTTCTGGGATCCGGTCTTCT	50.0	50.0	57.8	58.0	80-1377 bp	0.75	80.3
ndhE-ndhG_p3	GCATGCTCGAACACATTGAG	CTGGGATCCGGTCTTCTAGT	50.0	55.0	57.8	57.9	80-1374 bp	0.75	80.0
ndhE-ndhG_p4	GCATGCTCGAACACATTGAG	TGCGGCACAACTCCTTATTT	50.0	45.0	57.8	58.1	80-1240 bp	0.75	79.9
ndhE-ndhG_p5	GCATGCTCGAACACATTGAG	GTCATGGTACGGGGTTGTTT	50.0	50.0	57.8	58.1	80-1063 bp	0.75	79.8