Hotspot

ndhD-psaC

SSC · 117171-117312 · 142 bp

Back to Cochlearia

Pi 0.25

MarkerSeek score 73.70

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 142
variable_sites: 53
pi: 0.25
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	TTTCTTTCCACCCCACATGG	CTCAATGTGTTCGAGCATGC	50.0	50.0	58.0	57.8	80-843 bp	0.75	80.6
ndhD-psaC_p2	ACAGACGTTTCTTTCCACCC	CTCAATGTGTTCGAGCATGC	50.0	50.0	58.0	57.8	80-850 bp	0.75	80.3
ndhD-psaC_p3	CGAACCGGCAGATATTGGAA	CTCAATGTGTTCGAGCATGC	50.0	50.0	58.1	57.8	80-423 bp	0.75	80.2
ndhD-psaC_p4	CACCCCACATGGCTAAAAGT	CTCAATGTGTTCGAGCATGC	50.0	50.0	58.1	57.8	80-835 bp	0.75	80.0
ndhD-psaC_p5	TTTCTTTCCACCCCACATGG	AAGAACCGAGGACTGTGTTG	50.0	50.0	58.0	57.8	80-758 bp	0.75	80.0