Hotspot

rpl32-trnL

SSC · 113288-114247 · 960 bp

Back to Cochlearia

Pi 0.25

MarkerSeek score 82.20

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 960
variable_sites: 360
pi: 0.25
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	TCGCTCGATATGGAGTACGA	TGCAGACTGAGGCATTTTGT	50.0	45.0	57.8	58.0	80-1793 bp	0.75	80.6
rpl32-trnL_p2	TCGCTCGATATGGAGTACGA	TCGAACCGAGATGCTTTAGC	50.0	50.0	57.8	58.1	80-1283 bp	0.75	80.1
rpl32-trnL_p3	TCGCTCGATATGGAGTACGA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.8	57.9	80-1257 bp	0.75	79.7
rpl32-trnL_p4	TCGCTCGATATGGAGTACGA	AGCAGCGTGTCTACCAATTT	50.0	45.0	57.8	57.8	80-1250 bp	0.75	79.2
rpl32-trnL_p5	TCGCTCGATATGGAGTACGA	CTAAGAGCAGCGTGTCTACC	50.0	55.0	57.8	57.8	80-1255 bp	0.75	79.2