Hotspot

rps4

LSC · 56906-57511 · 606 bp

Back to Coccinia

Pi 0.00165

MarkerSeek score 44.90

Primer available yes

Label rps4

Feature Metadata

label_name: rps4
feature_type: gene
region: LSC
length_bp: 606
variable_sites: 1
pi: 0.00165
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4_p1	ATTGGTTGGACGTGCCTATC	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.0	57.9	1451-1455 bp	1.0	80.0
rps4_p2	CGGTTTCTGGGCCATAATCA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.9	57.9	1395-1399 bp	1.0	80.0
rps4_p3	AGGCTTTTGTTTACCGAGGG	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	1321-1325 bp	1.0	79.7
rps4_p4	ATTGGTTGGACGTGCCTATC	TGGATCCGCCAAGTCATCTA	50.0	50.0	58.0	58.2	1189-1192 bp	1.0	79.4
rps4_p5	ATTAACTCGGTTTCTGGGCC	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.9	57.9	1402-1406 bp	1.0	79.4