Hotspot

rpl2

IRb · 7799-9288 · 1490 bp

Back to Coccinia

Pi NA

MarkerSeek score 36.00

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: IRb
length_bp: 1490
variable_sites: 0
pi: NA
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.00
markerseek_score: 36.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	CGCTTCAACCGGGTTATTCT	TGTTTGTAAAGACGAAGAAAGA	50.0	31.8	58.3	54.0	1596 bp	1.0	44.0
rpl2_p2	ATCATTACGCTTCAACCGGG	TGTTTGTAAAGACGAAGAAAGA	50.0	31.8	58.3	54.0	1603 bp	1.0	44.0
rpl2_p3	CGCTTCAACCGGGTTATTCT	TGTTTGTAAAGACGAAGAAAGAA	50.0	30.4	58.3	54.7	1596 bp	1.0	44.0
rpl2_p4	CGCTTCAACCGGGTTATTCT	TTGTTTGTAAAGACGAAGAAAGA	50.0	30.4	58.3	54.7	1597 bp	1.0	44.0
rpl2_p5	ATCATTACGCTTCAACCGGG	TGTTTGTAAAGACGAAGAAAGAA	50.0	30.4	58.3	54.7	1603 bp	1.0	44.0