Hotspot

rpl20-rps12

LSC · 80642-81432 · 791 bp

Back to Coccinia

Pi 0.00632
MarkerSeek score 62.40
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
791
variable_sites
5
pi
0.00632
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 CTTGAATGAGCCCCTCGAAA GAAATGTCACCAAATCGCCC 50.0 50.0 57.9 57.7 948 bp 1.0 77.3
rpl20-rps12_p2 TTGAATGAGCCCCTCGAAAG GAAATGTCACCAAATCGCCC 50.0 50.0 57.9 57.7 947 bp 1.0 77.3
rpl20-rps12_p3 TTTGTTCTACGTCTCCGAGC GAAATGTCACCAAATCGCCC 50.0 50.0 57.7 57.7 906 bp 1.0 75.5
rpl20-rps12_p4 TGTTCTACGTCTCCGAGCTA GAAATGTCACCAAATCGCCC 50.0 50.0 57.6 57.7 904 bp 1.0 75.1
rpl20-rps12_p5 TTGTTCTACGTCTCCGAGCT GAAATGTCACCAAATCGCCC 50.0 50.0 58.5 57.7 905 bp 1.0 74.5