Hotspot

rbcL-accD

LSC · 68333-69013 · 681 bp

Back to Coccinia

Pi 0.00588

MarkerSeek score 62.30

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 681
variable_sites: 4
pi: 0.00588
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGTCCTGAACTAGCTGCTG	CTCCCCGTTAGACAACATCG	55.0	55.0	57.7	58.1	799-824 bp	1.0	77.5
rbcL-accD_p2	GCTGCTGCTTGTGAAGTATG	CTCCCCGTTAGACAACATCG	50.0	55.0	57.5	58.1	786-811 bp	1.0	75.6
rbcL-accD_p3	GAGTCCTGAACTAGCTGCTG	CCCCGTTAGACAACATCGAA	55.0	50.0	57.7	57.6	797-822 bp	1.0	75.2
rbcL-accD_p4	GAGTCCTGAACTAGCTGCTG	TCCCCGTTAGACAACATCGA	55.0	50.0	57.7	58.5	798-823 bp	1.0	74.5
rbcL-accD_p5	CTTGCTCGTGAGGGTAATGA	CTCCCCGTTAGACAACATCG	50.0	55.0	57.3	58.1	843-868 bp	1.0	74.5