Hotspot

rps4-trnT

LSC · 57512-57834 · 323 bp

Back to Coccinia

Pi 0.0377

MarkerSeek score 62.70

Primer available yes

Label rps4-trnT

Feature Metadata

label_name: rps4-trnT
feature_type: igs
region: LSC
length_bp: 323
variable_sites: 12
pi: 0.0377
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT_p1	GCCCTCTGTAACGCGACAT	CTCAGAGGTTAGAGCATCGC	57.9	55.0	60.2	57.9	401-405 bp	1.0	57.1
rps4-trnT_p2	CCCTCTGTAACGCGACAT	CTCAGAGGTTAGAGCATCGC	55.6	55.0	56.8	57.9	400-404 bp	1.0	56.5
rps4-trnT_p3	GCCCTCTGTAACGCGACAT	ATGGTCATCGGTTCGATTCC	57.9	50.0	60.2	57.8	369-373 bp	1.0	56.5
rps4-trnT_p4	GCCCTCTGTAACGCGACAT	CTGTTATGTGAGCCCGCTTA	57.9	50.0	60.2	57.7	422-426 bp	1.0	55.5
rps4-trnT_p5	GCCCTCTGTAACGCGACAT	TGTTATGTGAGCCCGCTTAG	57.9	50.0	60.2	57.7	421-425 bp	1.0	55.5