Hotspot

ycf3-trnS

LSC · 55914-56525 · 612 bp

Back to Coccinia

Pi 0.0114

MarkerSeek score 57.80

Primer available yes

Label ycf3-trnS

Feature Metadata

label_name: ycf3-trnS
feature_type: igs
region: LSC
length_bp: 612
variable_sites: 7
pi: 0.0114
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS_p1	CCTCTTTTTCCCCGGAAGTT	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.7	58.1	785-786 bp	1.0	77.7
ycf3-trnS_p2	CCTCTTTTTCCCCGGAAGTT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.7	58.2	806-807 bp	1.0	77.2
ycf3-trnS_p3	CCTCTTTTTCCCCGGAAGTT	TTCCAATGCTACGCCTTCAA	50.0	45.0	57.7	57.8	756-757 bp	1.0	76.9
ycf3-trnS_p4	ATCCCACCAGATCTAGGCAT	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.6	58.1	699-700 bp	1.0	76.6
ycf3-trnS_p5	CCTCTTTTTCCCCGGAAGTT	AGTTCCAATGCTACGCCTTC	50.0	50.0	57.7	58.3	758-759 bp	1.0	76.3