Hotspot

psaA-ycf3

LSC · 53210-53915 · 706 bp

Back to Coccinia

Pi 0.017

MarkerSeek score 55.70

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 706
variable_sites: 12
pi: 0.017
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CTTCTGGTTCCGGCGAAC	ATCAAGCCGCTGAGTATTGG	61.1	50.0	58.4	58.0	832-836 bp	1.0	61.3
psaA-ycf3_p2	CTTCTGGTTCCGGCGAAC	AGCCGCTGAGTATTGGAAAC	61.1	50.0	58.4	58.3	828-832 bp	1.0	59.6
psaA-ycf3_p3	CTTCTGGTTCCGGCGAAC	CAAGCCGCTGAGTATTGGAA	61.1	50.0	58.4	58.3	830-834 bp	1.0	59.6
psaA-ycf3_p4	ACTTCTGGTTCCGGCGAA	ATCAAGCCGCTGAGTATTGG	55.6	50.0	58.9	58.0	833-837 bp	1.0	58.0
psaA-ycf3_p5	CTTCTGGTTCCGGCGAAC	CGGAGGCTTGGTTCAATCAA	61.1	50.0	58.4	58.5	847-851 bp	1.0	57.9