Hotspot

psbM-trnD

LSC · 40195-41290 · 1096 bp

Back to Coccinia

Pi 0.00548

MarkerSeek score 62.00

Primer available yes

Label psbM-trnD

Feature Metadata

label_name: psbM-trnD
feature_type: igs
region: LSC
length_bp: 1096
variable_sites: 6
pi: 0.00548
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD_p1	AGGCGGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	47.6	50.0	58.0	58.5	1220-1221 bp	1.0	68.4
psbM-trnD_p2	AGGCGGTAGGAACTAGAATGA	GTTCAATTGGTCAGAGCACC	47.6	50.0	58.0	57.0	1221-1222 bp	1.0	64.3
psbM-trnD_p3	GGCGGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	50.0	50.0	56.4	58.5	1219-1220 bp	1.0	64.1
psbM-trnD_p4	AGGCGGTAGGAACTAGAATG	TTCAATTGGTCAGAGCACCG	50.0	50.0	56.4	58.5	1220-1221 bp	1.0	64.1
psbM-trnD_p5	AGGCGGTAGGAACTAGAATGA	TCAATTGGTCAGAGCACCG	47.6	52.6	58.0	57.8	1219-1220 bp	1.0	62.4