Hotspot

trnC-petN

LSC · 38208-39099 · 892 bp

Back to Coccinia

Pi 0.0045

MarkerSeek score 61.70

Primer available yes

Label trnC-petN

Feature Metadata

label_name: trnC-petN
feature_type: igs
region: LSC
length_bp: 892
variable_sites: 4
pi: 0.0045
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-petN_p1	TTTTGTATCGTTTTGGCGGC	AGAGTCCACTTCTTCCCCAT	45.0	50.0	57.9	57.6	1066-1069 bp	1.0	77.3
trnC-petN_p2	TTTTGTATCGTTTTGGCGGC	TCTTCCCCATACTACGAGGG	45.0	55.0	57.9	57.6	1056-1059 bp	1.0	77.1
trnC-petN_p3	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	1009-1012 bp	1.0	77.1
trnC-petN_p4	TTTCCCCAGTTCAAATCCGG	TCTTCCCCATACTACGAGGG	50.0	55.0	58.1	57.6	999-1002 bp	1.0	76.9
trnC-petN_p5	TGCAAATCCCTTTTTCCCCA	AGAGTCCACTTCTTCCCCAT	45.0	50.0	57.9	57.6	1021-1024 bp	1.0	76.9