Hotspot

trnK-rps16

LSC · 13566-14312 · 747 bp

Back to Coccinia

Pi 0.011

MarkerSeek score 67.90

Primer available yes

Label trnK-rps16

Feature Metadata

label_name: trnK-rps16
feature_type: igs
region: LSC
length_bp: 747
variable_sites: 8
pi: 0.011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-rps16_p1	AGTCGCGGTCTTACAAACTC	GGTGCTCAACCTACAGGAAC	50.0	55.0	57.9	58.2	922-942 bp	1.0	80.6
trnK-rps16_p2	GTCGCGGTCTTACAAACTCT	GGTGCTCAACCTACAGGAAC	50.0	55.0	57.9	58.2	921-941 bp	1.0	80.6
trnK-rps16_p3	GTCGCGGTCTTACAAACTCT	AAGGCAGGTGTTTTTACGGA	50.0	45.0	57.9	57.6	882-902 bp	1.0	79.4
trnK-rps16_p4	GTCGCGGTCTTACAAACTCT	AGGCAGGTGTTTTTACGGAA	50.0	45.0	57.9	57.6	881-901 bp	1.0	79.4
trnK-rps16_p5	CAGTCGCGGTCTTACAAACT	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.2	58.2	923-943 bp	1.0	79.3