Hotspot

ycf1-ndhF

SSC · 114035-114082 · 48 bp

Back to Cnidoscolus

Pi 0.222

MarkerSeek score 67.10

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 48
variable_sites: 6
pi: 0.222
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.56
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	GATCCGGACAAAATCGACGA	AATATGTAGGAAGCGGTCGC	50.0	50.0	58.1	57.8	849-878 bp	1.0	82.4
ycf1-ndhF_p2	CGGACAAAATCGACGAAACG	AATATGTAGGAAGCGGTCGC	50.0	50.0	58.2	57.8	845-874 bp	1.0	81.9
ycf1-ndhF_p3	TCCGGACAAAATCGACGAAA	AATATGTAGGAAGCGGTCGC	45.0	50.0	57.8	57.8	847-876 bp	1.0	81.9
ycf1-ndhF_p4	GGACAAAATCGACGAAACGG	AATATGTAGGAAGCGGTCGC	50.0	50.0	57.8	57.8	844-873 bp	1.0	81.3
ycf1-ndhF_p5	GATCCGGACAAAATCGACGA	TACGAAATATGACGTGGCCC	50.0	50.0	58.1	57.8	1455-1484 bp	1.0	80.1