Hotspot

petA-psbJ

LSC · 64315-65419 · 1105 bp

Back to Cnidoscolus

Pi 0.00181

MarkerSeek score 59.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1105
variable_sites: 2
pi: 0.00181
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCCGTTATTTTGGCAC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.1	57.6	1216-1238 bp	1.0	76.6
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	1233-1255 bp	1.0	76.0
petA-psbJ_p3	CATTACGTGTCCAAGGCCTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1247-1269 bp	1.0	75.8
petA-psbJ_p4	GACCCATTACGTGTCCAAGG	ATTCCGGATTGGGTTCATCC	55.0	50.0	58.3	57.6	1251-1273 bp	1.0	75.1
petA-psbJ_p5	TGTTCTTCTTGGCATCCGTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.7	57.6	1226-1248 bp	1.0	75.0