Hotspot

ycf2

IRb · 86980-93300 · 6321 bp

Back to Cnidium

Pi 0.00972

MarkerSeek score 49.80

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRb
length_bp: 6321
variable_sites: 61
pi: 0.00972
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	ACTATTGGCTGAATCCCGTG	GATACGGGCCTTTTCCACAT	50.0	50.0	57.7	57.9	155-161 bp	1.0	84.5
ycf2_p2	ACTATTGGCTGAATCCCGTG	TTGATACGGGCCTTTTCCAC	50.0	50.0	57.7	58.2	157-163 bp	1.0	83.5
ycf2_p3	CTATTGGCTGAATCCCGTGA	GATACGGGCCTTTTCCACAT	50.0	50.0	57.4	57.9	154-160 bp	1.0	82.2
ycf2_p4	GACTATTGGCTGAATCCCGT	GATACGGGCCTTTTCCACAT	50.0	50.0	57.4	57.9	156-162 bp	1.0	82.1
ycf2_p5	CTATTGGCTGAATCCCGTGA	TTGATACGGGCCTTTTCCAC	50.0	50.0	57.4	58.2	156-162 bp	1.0	81.2