Hotspot

psbA

LSC · 361-1422 · 1062 bp

Back to Cnidium

Pi 0.00377

MarkerSeek score 48.10

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 4
pi: 0.00377
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	ACTCGTATATACTAAAACCCGGTC	AAATTCGTGTGCTTGGGAGT	41.7	45.0	57.7	58.0	1137 bp	1.0	45.6
psbA_p2	ACTCGTATATACTAAAACCCGGTC	TGCTTGGGAGTCCCTGATAA	41.7	50.0	57.7	58.0	1128 bp	1.0	45.5
psbA_p3	ACTCGTATATACTAAAACCCGGT	AAATTCGTGTGCTTGGGAGT	39.1	45.0	56.6	58.0	1137 bp	1.0	45.2
psbA_p4	ACTCGTATATACTAAAACCCGGT	TGCTTGGGAGTCCCTGATAA	39.1	50.0	56.6	58.0	1128 bp	1.0	45.0
psbA_p5	AACTCGTATATACTAAAACCCGGT	AAATTCGTGTGCTTGGGAGT	37.5	45.0	57.3	58.0	1138 bp	1.0	42.4