Hotspot

ndhF

SSC · 111730-113958 · 2229 bp

Back to Cnidium

Pi 0.0104

MarkerSeek score 49.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2229
variable_sites: 23
pi: 0.0104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CTCATGAAGGCCTACCGTTG	GGGGGTAGGATCCGGTAAAT	55.0	55.0	58.3	58.3	2380-2404 bp	1.0	77.4
ndhF_p2	GGCCTACTAAACGTAGTCGC	GGGGGTAGGATCCGGTAAAT	55.0	55.0	58.2	58.3	2338 bp	0.5	56.6
ndhF_p3	GTCGCAAAGCAAGTGTCAAA	GGGGGTAGGATCCGGTAAAT	45.0	55.0	57.8	58.3	2323 bp	0.5	56.4
ndhF_p4	TAGTCGCAAAGCAAGTGTCA	GGGGGTAGGATCCGGTAAAT	45.0	55.0	57.8	58.3	2325 bp	0.5	56.0
ndhF_p5	AACGTAGTCGCAAAGCAAGT	GGGGGTAGGATCCGGTAAAT	45.0	55.0	58.4	58.3	2329 bp	0.5	54.5