Hotspot

ndhF-rpl32

SSC · 113959-115010 · 1052 bp

Back to Cnidium

Pi 0.0201

MarkerSeek score 61.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1052
variable_sites: 20
pi: 0.0201
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGACGGAAAAAGAAG	TGACAAACAGGTTGATGGGG	50.0	50.0	58.0	58.0	1571-1576 bp	1.0	84.1
ndhF-rpl32_p2	AAGCGGGTCGATCAAGTAAC	TGACAAACAGGTTGATGGGG	50.0	50.0	58.0	58.0	1749-1754 bp	1.0	84.0
ndhF-rpl32_p3	TGCCGACGGAAAAAGAAGAA	TGACAAACAGGTTGATGGGG	45.0	50.0	58.0	58.0	1569-1574 bp	1.0	84.0
ndhF-rpl32_p4	TTGCCGACGGAAAAAGAAGA	TGACAAACAGGTTGATGGGG	45.0	50.0	58.0	58.0	1570-1575 bp	1.0	84.0
ndhF-rpl32_p5	CCGTGTGAACCAAAACCCTA	TGACAAACAGGTTGATGGGG	50.0	50.0	58.0	58.0	1977-1982 bp	1.0	83.3