Hotspot

psbE-petL

LSC · 65407-66405 · 999 bp

Back to Cnidium

Pi 0.0142

MarkerSeek score 53.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 999
variable_sites: 14
pi: 0.0142
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AAAGGATCAAAACGGCCAGT	GGTTACCGTTAAAGCCGCTA	45.0	50.0	58.0	58.0	1263-1271 bp	1.0	81.8
psbE-petL_p2	AAGGATCAAAACGGCCAGTT	GGTTACCGTTAAAGCCGCTA	45.0	50.0	58.0	58.0	1262-1270 bp	1.0	81.8
psbE-petL_p3	TCGTTCGGGTTTGATTGTGT	GGTTACCGTTAAAGCCGCTA	45.0	50.0	58.0	58.0	1471-1479 bp	1.0	81.3
psbE-petL_p4	AAAGGATCAAAACGGCCAGT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	1775-1783 bp	1.0	81.2
psbE-petL_p5	AAGGATCAAAACGGCCAGTT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	1774-1782 bp	1.0	81.2