Hotspot

trnS-GCU-trnG-UCC

LSC · 8561-9133 · 573 bp

Back to Cnidium

Pi 0.0196

MarkerSeek score 60.00

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 573
variable_sites: 11
pi: 0.0196
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	GGGGGTTACTCGAACGAATC	45.0	55.0	58.0	58.1	649-660 bp	1.0	81.8
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	TAAGGGGGTTACTCGAACGA	45.0	50.0	58.0	57.8	652-663 bp	1.0	80.7
trnS-GCU-trnG-UCC_p3	ATTAGCAATCCGCCGCTTTA	AGGGGGTTACTCGAACGAAT	45.0	50.0	58.0	58.4	650-661 bp	1.0	78.9
trnS-GCU-trnG-UCC_p4	GCTTTAGTCCACTCAGCCAT	GGGGGTTACTCGAACGAATC	50.0	55.0	57.6	58.1	635-646 bp	1.0	78.9
trnS-GCU-trnG-UCC_p5	AGTCCACTCAGCCATCTCTC	GGGGGTTACTCGAACGAATC	55.0	55.0	58.5	58.1	630-641 bp	1.0	78.1