Hotspot

trnH-GUG-psbA

LSC · 155-360 · 206 bp

Back to Cnidium

Pi 0.0777

MarkerSeek score 72.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 206
variable_sites: 16
pi: 0.0777
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	AGCTCCATCTACAAATGGGT	55.0	45.0	58.1	56.2	250-262 bp	1.0	69.5
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	AGCTCCATCTACAAATGGGT	50.0	45.0	57.9	56.2	264-276 bp	1.0	69.2
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	AGCTCCATCTACAAATGGGT	50.0	45.0	57.9	56.2	263-275 bp	1.0	69.2
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	AGCTCCATCTACAAATGGGT	50.0	45.0	57.9	56.2	265-277 bp	1.0	69.2
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	AAGCTCCATCTACAAATGGGT	55.0	42.9	58.1	57.0	251-263 bp	1.0	67.7