Hotspot

ndhD

SSC · 118482-119999 · 1518 bp

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Pi 0.00477
MarkerSeek score 38.30
Primer available yes
Label ndhD

Feature Metadata

label_name
ndhD
feature_type
gene
region
SSC
length_bp
1518
variable_sites
17
pi
0.00477
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
38.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD_p1 AGACTTTGCTTTCTGTCTCA TGGTCAAAACGTATCTTGTCT 40.0 38.1 54.5 55.2 1564 bp 0.857143 35.2
ndhD_p2 AAGACTTTGCTTTCTGTCTCA TGGTCAAAACGTATCTTGTCT 38.1 38.1 55.4 55.2 1565 bp 0.857143 35.2
ndhD_p3 AGACTTTGCTTTCTGTCTCA TGGTCAAAACGTATCTTGTCTT 40.0 36.4 54.5 56.0 1564 bp 0.857143 35.2
ndhD_p4 AGACTTTGCTTTCTGTCTCA TTGGTCAAAACGTATCTTGTCT 40.0 36.4 54.5 56.0 1565 bp 0.857143 35.2
ndhD_p5 AAGACTTTGCTTTCTGTCTCA TGGTCAAAACGTATCTTGTCTT 38.1 36.4 55.4 56.0 1565 bp 0.857143 35.2