Hotspot

rrn5-rrn4.5

IRa · 133723-133939 · 217 bp

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Pi 0.0132
MarkerSeek score 53.60
Primer available yes
Label rrn5-rrn4.5

Feature Metadata

label_name
rrn5-rrn4.5
feature_type
igs
region
IRa
length_bp
217
variable_sites
10
pi
0.0132
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn5-rrn4.5_p1 AGTATCGTCACCGCAGTAGA CAGCTGAGGCATCCTAACAG 50.0 55.0 58.0 58.0 333-338 bp 1.0 82.9
rrn5-rrn4.5_p2 TATCGTCACCGCAGTAGAGT CAGCTGAGGCATCCTAACAG 50.0 55.0 58.0 58.0 331-336 bp 1.0 82.9
rrn5-rrn4.5_p3 TATCGTCACCGCAGTAGAGT GCTGAGGCATCCTAACAGAC 50.0 55.0 58.0 58.1 329-334 bp 1.0 82.9
rrn5-rrn4.5_p4 AGTATCGTCACCGCAGTAGA GTCAAGTGGAAGTGCAGTGA 50.0 50.0 58.0 58.0 359-364 bp 1.0 82.7
rrn5-rrn4.5_p5 TATCGTCACCGCAGTAGAGT GTCAAGTGGAAGTGCAGTGA 50.0 50.0 58.0 58.0 357-362 bp 1.0 82.7