Hotspot

ccsA-ndhD

SSC · 118257-118481 · 225 bp

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Pi 0.0349
MarkerSeek score 64.90
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
225
variable_sites
16
pi
0.0349
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 CGAATTCGGCACTTGTAGCT TCCGGATTTCGTTCTTTCACT 50.0 42.9 58.6 57.6 429-430 bp 1.0 66.7
ccsA-ndhD_p2 ATTCGGCACTTGTAGCTTCT TCCGGATTTCGTTCTTTCACT 45.0 42.9 57.2 57.6 426-427 bp 1.0 65.7
ccsA-ndhD_p3 ACGAATTCGGCACTTGTAGC TCCGGATTTCGTTCTTTCACT 50.0 42.9 58.9 57.6 430-431 bp 1.0 64.4
ccsA-ndhD_p4 AATTCGGCACTTGTAGCTTCT TCCGGATTTCGTTCTTTCACT 42.9 42.9 57.9 57.6 427-428 bp 1.0 63.4
ccsA-ndhD_p5 CGAATTCGGCACTTGTAGCT TCCGGATTTCGTTCTTTCAC 50.0 45.0 58.6 56.1 429-430 bp 1.0 62.6