Hotspot

rpl32-trnL-UAG

SSC · 116317-117132 · 816 bp

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Pi 0.0102
MarkerSeek score 52.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
816
variable_sites
24
pi
0.0102
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGCAAAGTCAGTTTCTACCGG TCCTAAGAGCAGCGTGTCTA 47.6 50.0 58.2 57.9 927-948 bp 1.0 71.3
rpl32-trnL-UAG_p2 GCAAAGTCAGTTTCTACCGGA TCCTAAGAGCAGCGTGTCTA 47.6 50.0 58.2 57.9 926-947 bp 1.0 71.2
rpl32-trnL-UAG_p3 GCAAAGTCAGTTTCTACCGG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 56.7 57.9 926-947 bp 1.0 71.1
rpl32-trnL-UAG_p4 AGCAAAGTCAGTTTCTACCGG AGCAGCGTGTCTACCAATTT 47.6 45.0 58.2 57.8 920-941 bp 1.0 70.8
rpl32-trnL-UAG_p5 GCAAAGTCAGTTTCTACCGGA AGCAGCGTGTCTACCAATTT 47.6 45.0 58.2 57.8 919-940 bp 1.0 70.8