Hotspot

rrn4.5-rrn5

IRb · 110447-110663 · 217 bp

Back to Clivia

Pi 0.0132
MarkerSeek score 53.60
Primer available yes
Label rrn4.5-rrn5

Feature Metadata

label_name
rrn4.5-rrn5
feature_type
igs
region
IRb
length_bp
217
variable_sites
10
pi
0.0132
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn4.5-rrn5_p1 CAGCTGAGGCATCCTAACAG AGTATCGTCACCGCAGTAGA 55.0 50.0 58.0 58.0 333-338 bp 1.0 82.9
rrn4.5-rrn5_p2 CAGCTGAGGCATCCTAACAG TATCGTCACCGCAGTAGAGT 55.0 50.0 58.0 58.0 331-336 bp 1.0 82.9
rrn4.5-rrn5_p3 GCTGAGGCATCCTAACAGAC TATCGTCACCGCAGTAGAGT 55.0 50.0 58.1 58.0 329-334 bp 1.0 82.9
rrn4.5-rrn5_p4 GTCAAGTGGAAGTGCAGTGA AGTATCGTCACCGCAGTAGA 50.0 50.0 58.0 58.0 359-364 bp 1.0 82.7
rrn4.5-rrn5_p5 GTCAAGTGGAAGTGCAGTGA TATCGTCACCGCAGTAGAGT 50.0 50.0 58.0 58.0 357-362 bp 1.0 82.7