Hotspot

rps12-trnV-GAC

IRb · 101413-103223 · 1811 bp

Back to Clivia

Pi 0.00337
MarkerSeek score 51.60
Primer available yes
Label rps12-trnV-GAC

Feature Metadata

label_name
rps12-trnV-GAC
feature_type
igs
region
IRb
length_bp
1811
variable_sites
14
pi
0.00337
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-trnV-GAC_p1 TGGCAACTTTACGTAAGGCA CGGGGGAGCAAGTCAAAATA 45.0 50.0 57.7 57.9 1942-1968 bp 1.0 78.6
rps12-trnV-GAC_p2 TGGCAACTTTACGTAAGGCA GATGACTTCCACCACGTCAA 45.0 50.0 57.7 57.8 1881-1907 bp 1.0 78.4
rps12-trnV-GAC_p3 TGGCAACTTTACGTAAGGCA AACTGATGACTTCCACCACG 45.0 50.0 57.7 57.8 1885-1911 bp 1.0 78.3
rps12-trnV-GAC_p4 TGGCAACTTTACGTAAGGCA ATGACTTCCACCACGTCAAG 45.0 50.0 57.7 57.8 1880-1906 bp 1.0 78.3
rps12-trnV-GAC_p5 TGGCAACTTTACGTAAGGCA CTGATGACTTCCACCACGTC 45.0 55.0 57.7 58.3 1883-1909 bp 1.0 77.4