Hotspot

rps18-rpl20

LSC · 70704-70964 · 261 bp

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Pi 0.0197
MarkerSeek score 41.60
Primer available yes
Label rps18-rpl20

Feature Metadata

label_name
rps18-rpl20
feature_type
igs
region
LSC
length_bp
261
variable_sites
12
pi
0.0197
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
41.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps18-rpl20_p1 TGGTCCTAGAACCAGAAATA AGAATTGTCTTTACATGATTTCCA 40.0 29.2 52.6 54.8 327-333 bp 1.0 43.4
rps18-rpl20_p2 TGGTCCTAGAACCAGAAATAA AGAATTGTCTTTACATGATTTCCA 38.1 29.2 53.6 54.8 327-333 bp 1.0 43.4
rps18-rpl20_p3 TGGTCCTAGAACCAGAAATAAA AGAATTGTCTTTACATGATTTCCA 36.4 29.2 54.4 54.8 327-333 bp 1.0 43.4
rps18-rpl20_p4 TGGTCCTAGAACCAGAAATA AAGAATTGTCTTTACATGATTTCCA 40.0 28.0 52.6 55.5 328-334 bp 1.0 43.4
rps18-rpl20_p5 TGGTCCTAGAACCAGAAATA AGAATTGTCTTTACATGATTTCCAA 40.0 28.0 52.6 55.5 327-333 bp 1.0 43.4